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| Registros recuperados: 152 | |
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| Radhika,M.; Saugata,Majumder; Murali,H.S.; Batra,H.V.. |
| Salmonella enterica and Shigella species are commonly associated with food and water borne infections leading to gastrointestinal diseases. The present work was undertaken to develop a sensitive and reliable PCR based detection system for simultaneous detection of Salmonella enterica and Shigella at species level. For this the conserved regions of specific genes namely ipaH1, ipaH, wbgZ, wzy and invA were targeted for detection of Shigella genus, S. flexneri, S. sonnei, S. boydii and Salmonella enterica respectively along with an internal amplification control (IAC). The results showed that twenty Salmonella and eleven Shigella spp., were accurately identified by the assay without showing non-specificity against closely related other Enterobacteriaceae... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Shigella; Multiplex PCR; Spiking. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200041 |
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| Possebon,Fabio Sossai; Costa,Luis Felipe Zuccolo Paschoal da; Yamatogi,Ricardo Seiti; Rodrigues,Marianna Vaz; Sudano,Mateus José; Pinto,José Paes de Almeida Nogueira. |
| A pesquisa de Salmonella em carcaças de aves tem mostrado resultados discrepantes, dependendo se as amostras foram colhidas ainda na indústria, imediatamente após o chiller ou no comércio varejista, quando se encontram submetidas à refrigeração por vários dias. Técnicas mais sensíveis, tais como a Reação em Cadeia pela Polimerase (PCR), podem fornecer dados importantes sobre o efeito do resfriamento sobre as células do patógeno, comparando seus resultados com os da metodologia microbiológica convencional (MC). Foram colhidas 130 carcaças de frango, sendo que 65 foram obtidas ainda na indústria (pós-chiller) e imediatamente analisadas e 65 carcaças embaladas e estocadas a 5°C por 72 horas (simulando o varejo), sendo então realizada a pesquisa do patógeno... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Salmonella; Frango; PCR; Refrigeração. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000100021 |
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| Morales Cabrera, Mercedes. |
| En este estudio se evaluó la actividad antimicrobiana de los extractos etanólicos, metanólicos y acuosos (frío y caliente) de cálices deshidratados de Hibiscus sabdariffa L. de cinco genotipos, sobre S. typhimurium (ATCC14028) y S. cholleraesuis (ATCC10708), se empleó el método difusión en agar. Se determinó la concentración mínima inhibitoria (CMI) y la concentración mínima bactericida (CMB) por dilución. Se analizó la influencia del pH en la actividad antimicrobiana. Así mismo se realizó la caracterización fenólica de los extractos etanólicos. El efecto antimicrobiano fue variable; los extractos de la variedad Alma blanca (cálices verdes), presentaron el mayor efecto antimicrobiano (diámetros de inhibición de12.5 a 19.5 mm) sobre ambos serotipos de... |
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Palavras-chave: CMI; CMB; PH; Salmonella; Compuestos fenólicos; HPLC; MIC; MCB; Phenolic compounds; Fitopatología; Maestría. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/568 |
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| Morales Cabrera, Mercedes. |
| En este estudio se evaluó la actividad antimicrobiana de los extractos etanólicos, metanólicos y acuosos (frío y caliente) de cálices deshidratados de Hibiscus sabdariffa L. de cinco genotipos, sobre S. typhimurium (ATCC14028) y S. cholleraesuis (ATCC10708), se empleó el método difusión en agar. Se determinó la concentración mínima inhibitoria (CMI) y la concentración mínima bactericida (CMB) por dilución. Se analizó la influencia del pH en la actividad antimicrobiana. Así mismo se realizó la caracterización fenólica de los extractos etanólicos. El efecto antimicrobiano fue variable; los extractos de la variedad Alma blanca (cálices verdes), presentaron el mayor efecto antimicrobiano (diámetros de inhibición de12.5 a 19.5 mm) sobre ambos serotipos de... |
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Palavras-chave: CMI; CMB; PH; Salmonella; Compuestos fenólicos; HPLC; MIC; MCB; Phenolic compounds; Fitopatología; Maestría. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/568 |
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| Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger; Carvalho,Ana Lyl de; Brandelli,Adriano. |
| The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022 |
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| Voss-Rech,Daiane; Klein,Cátia Silene; Techio,Vânia Helena; Scheuermann,Gerson Neudi; Rech,Gilberto; Fiorentin,Laurimar. |
| in vitro antibacterial activity of 21 hydroethanolic vegetal extracts was assessed against 20 serovars of Salmonella. Regarding the tested extracts, 85.7% of them presented antibacterial activity. The six active extracts which showed activity on the largest number of serovars and the extract of Eucalyptus sp. were submitted to the determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Of these, six extracts showed bacteriostatic and bactericidal activity with MIC and MBC for Punica granatum (pomegranate) from 20 and 60mg mL-1, for Eugenia jambolana (rose apple) from 40 and 240mg mL-1, Eugenia uniflora (surinam cherry) from 80 and 240mg mL-1, Caryophyllus aromaticus (clove) from 10 and 60mg mL-1, Psidium araca... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibacterial activity; Vegetal extracts; Salmonella. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200022 |
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| Indu,M.N.; Hatha,A.A.M.; Abirosh,C.; Harsha,U.; Vivekanandan,G.. |
| Antibacterial activity of extracts of Allium sativum (garlic), Myristica fragrans (nutmeg), Zingiber officinale (ginger), Allium cepa (onion) and Piper nigrum (pepper) has been evaluated against 20 different serogroups of Escherichia coli, 8 serotypes of Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Garlic extract showed excellent antibacterial activity against all the test organisms, except L. monocytogenes. Nutmeg showed good anti-listerial activity, although activity against E. coli and Salmonella were serotype dependent. Both garlic and nutmeg extracts were effective against A. hydrophila. Extracts of ginger showed inhibitory activity against two serogroups of E. coli: as O8 (enterotoxigenic E. coli) and O88 only. Extracts of onion and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spices extract; Antibacterial activity; Escherichia coli; Salmonella; Listeria monocytogenes; Aeromonas hydrophila. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200011 |
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| Villafañe,Isabel E. Gómez; Miñarro,Fernando; Ribicich,Mabel; Rossetti,Carlos A.; Rossotti,Daniel; Busch,María. |
| We have studied the prevalence of Trichinella spiralis, Leptospira spp. and Salmonella spp. in rats and opossums that inhabit poultry farms of Exaltación de la Cruz, Buenos Aires, Argentina, to determine the potential sanitary risk for humans that are in contact with these animals. The study was carried out on 48 poultry farms between spring 1999 and winter 2001. The study of opossums began in winter 2000. During the study period we captured 152 Rattus norvegicus, 3 Rattus rattus, 16 Didelphis albiventris and 1 Lutreolina crassicaudata. We have registered the presence of rats and opossums in 70% and 27% of the studied farms, respectively. The percentage of farms with rats was independent of the presence or absence of pigs. We did not detect the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zoonosis; Salmonella; Poultry farms; Rats; Opossum. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300017 |
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| Santurio,Janio Morais; Santurio,Deise Flores; Pozzatti,Patrícia; Moraes,Cristiane; Franchin,Paulo Rogério; Alves,Sydney Hartz. |
| Avaliou-se a atividade antimicrobiana dos óleos essenciais de orégano (Origanum vulgare), tomilho (Thymus vulgaris) e canela (Cinnamomum zeylanicum) frente a 60 amostras de Salmonella enterica distribuídas entre 20 sorovares, todos isolados de carcaças de aves. A concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM) foram determinadas para cada isolado, utilizando-se a técnica de microdiluição em caldo. O óleo essencial de orégano evidenciou forte atividade antibacteriana (CIM média = 529mig ml-1 e CBM média= 661mig ml-1), seguido do tomilho (CIM média=961mig ml-1 e CBM média= 1074mig ml-1), com atividade moderada, enquanto que a menor atividade foi observada com o óleo essencial de canela (CIM média= 1335mig ml-1 e CBM média =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Óleos essenciais; Orégano; Tomilho; Canela; Atividade antimicribiana. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000300031 |
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| Barros,M.R.; Andreatti Filho,R.L.; Lima,E.T.; Crocci,J.A.. |
| Inglúvios e cecos de reprodutoras comerciais de frangos de corte foram utilizados para o isolamento de Lactobacillus spp. As estirpes isoladas foram caracterizadas como Gram-positivo, catalase negativo, produtoras de gás em glicose, não produtoras de H2S em triple sugar iron e identificadas pela reação em cadeia da polimerase como Lactobacillus reuteri e Lactobacillus salivarius. A utilização da técnica spot-on-the-lawn para avaliação da inibição in vitro permitiu a análise de vários microrganismos simultaneamente. Todas as estirpes isoladas inibiram in vitro S. Enteritidis fagotipo 4, S. Enteritidis fagotipo 28, S. Typhimurium, S. Pullorum, S. Agona, S. Anatum, S. Dublin e S. Senftenberg. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reprodutora de frango de corte; Identificação; Lactobacillus; Salmonella; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000400013 |
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| Garrido,Antonio Marin; Burgos,Mª Jose Grande; Márquez,Mª Luisa Fernández; Aguayo,Mª Carmen López; Pulido,Rubén Pérez; Árbol,Julia Toledo del; Gálvez,Antonio; López,Rosario Lucas. |
| Salmonella serovars sampled from meat products in Southern Spain (Andalucía) during the period 2002-2007 were analyzed in this study. The serovars most frequently detected (in order) were Typhimurium, Enteritidis, Derby, Anatum and Rissen. Isolates (n = 43) were tested for sensitivity to biocides, including the quaternary ammonium compounds benzalkonium chloride (BC), cetrimide (CT) and hexadecylpyridinium chloride (HDP), and the bisphenols triclosan (TC) and hexachlorophene (CF). The minimum inhibitory concentration (MIC) for the quaternary ammonium compounds was in the range of 25 to 50 mg/L for most isolates, although a few isolates required much higher concentrations, up to 250 mg/L. Bisphenols showed higher inhibitory activity, with a MIC of 2.5 to 25... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Biocides; Serovars; Meat; Chorizo. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401177 |
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| Landa Salgado, Patricia. |
| Durante las últimas dos décadas las infecciones por Salmonella se han registrado entre las enfermedades más frecuentes, afectando a personas de todas las edades y condiciones sociales y causando numerosas hospitalizaciones y muerte de personas por el consumo de frutas y hortalizas frescas. En frutas, en 2011 el Centro para el Control de Enfermedades y Prevención en EUA reportó un brote de infección por S. Agona asociado con el consumo de papaya fresca en el que 106 personas resultaron afectadas. Mientras que en hortalizas, S. typhimurium se ha aislado de diversas especies entre las que se encuentra el nopal verdura (Opuntia ficus-indica L. Mill) (Quiroz-Santiago, 2009; Hernández et. al. 2009). Debido a que el nopal se consume en fresco para el control de... |
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Palavras-chave: Salmonella; Nopal; Persistencia; Respuesta de defensa nopal; Persistence; Defense of nopal; Doctorado; Fitopatología. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/745 |
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| Martínez Martínez, Talina Olivia. |
| La calidad sanitaria en los productos hortofrutícolas debe considerarse una prioridad en la producción de alimentos. Actualmente en México, existe una gran preocupación por las asociaciones de productores y gobierno federal para adoptar sistemas de producción que reduzcan riesgos de contaminación (SRRC). Para la implementación de estos sistemas, es necesario cumplir con una serie de requisitos, entre ellos se encuentra la validación de procesos. Un método inmediato para efectuar este requerimiento, es la evaluación de la calidad sanitaria desde el punto de vista microbiológico y toxicológico, lo que hace necesario determinar un plan de muestreo y las técnicas apropiadas para la detección y cuantificación de patógenos indicadores. Con este propósito, en... |
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Palavras-chave: Salmonella; Escherichia coli; Pesticides; Phenolic compounds; Survival; Sample size; Entomología y Acarología; Doctorado. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/590 |
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| Taban,Birce Mercanoglu; Aytac,Sait Aykut; Akkoc,Nefise; Akcelik,Mustafa. |
| In the last decade, ready-to-eat (RTE) salad vegetables are gaining increasing importance in human diet. However, since they are consumed fresh, inadequate washing during processing can bring on some foodborne illnesses, like salmonellosis, since these food items have natural contamination from soil and water. During 2009-2010, a total of 81 samples were purchased arbitrarily from local markets in Ankara, and were examined for Salmonella contamination. Salmonella screening was performed by using anti-Salmonella magnetic beads system and polymerase chain reaction (PCR) identification of the suspected colonies. Then, the antibiotic resistance profiles of four Salmonella strains identified (strains RTE-1, RTE-2, RTE-3, and RTE-4) were also investigated, since... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contamination; Plasmid-mediated sulfonamide resistance; Ready-to-eat salad vegetables; Food safety; Salmonella. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200007 |
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| Ribeiro,Vinicius B.; Lincopan,Nilton; Landgraf,Mariza; Franco,Bernadete D.G.M.; Destro,Maria T.. |
| In recent years, an increase in the occurrence of antimicrobial resistance among Salmonella enterica has been observed in several countries, which is worrisome because S. enterica is one of the most common causes of human gastroenteritis worldwide. The aim of this study was to characterize class 1 integrons and antibiotic resistance genotypes in Salmonella enterica isolates recovered from foodstuff and related sources. Nineteen multidrug-resistant (MDR) Salmonella enterica isolates were recovered. Higher resistance rates to tetracycline (90%), streptomycin (80%), sulfamethoxazole-trimethoprim (80%), ampicillin (60%) and nalidixic acid (70%) were related to the presence of the tetA, aadA, sul1/sul2, blaTEM-1 genes, and a codon mutation at position 83 of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Multidrug-resistant; Class 1 integrons; Foodstuff; Brazil. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200033 |
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| Registros recuperados: 152 | |
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